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KMID : 0894520130170040299
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Development & Reproduction
2013 Volume.17 No. 4 p.299 ~ p.309
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PKCn Regulates the TGFB3-induced Chondrogenic Differentiation of Human Mesenchymal Stem Cell
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Ku Bo-Mi
Yune Young-Phil
Lee Eun-Shin
Hah Young-Sool
Park Jae-Yong
Jeong Joo-Yeon
Lee Dong-Hoon
Cho Gyeong-Jae
Choi Wan-Sung
Kang Sang-Soo
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Abstract
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Transforming growth factor (TGF) family is well known to induce the chondrogenic differentiation of mesenchymal stem cells (MSC). However, the precise signal transduction pathways and underlying factors are not well known. Thus the present study aims to evaluate the possible role of C2 domain in the chondrogenic differentiation of human mesenchymal stem cells. To this end, 145 C2 domains in the adenovirus were individually transfected to hMSC, and morphological changes were examined. Among 145 C2 domains, C2 domain of protein kinase C eta (PKCn) was selected as a possible chondrogenic differentiation factor for hMSC. To confirm this possibility, we treated TGFB3, a well known chondrogenic differentiation factor of hMSC, and examined the increased-expression of glycosaminoglycan (GAG), collagen type II (COL II) as well as PKCn using PT-PCR, immunocytochemistry and Western blot analysis. To further evaluation of C2 domain of PKCn, we examined morphological changes, expressions of GAG and COL II after transfection of PKCn-C2 domain in hMSC. Overexpression of PKCn-C2 domain induced morphological change and increased GAG and COL II expressions. The present results demonstrate that PKCn involves in the TGF-B3-induced chondrogenic differentiation of hMSC, and C2 domain of PKCn has important role in this process.
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KEYWORD
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Human mesenchymal stem cell, Chondrogenesis, TGF-B3, PKCn, C2-domain
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